学习经历

1995-1998  复旦大学遗传学研究所，博士

1992-1995  中国科学技术大学生物系，硕士

1987-1992  中国科学技术大学生物系，学士

主要工作经历与任职情况

1998-1999  美国佛蒙特大学（University of Vermont）医学院，博士后

1999-2006  美国宾夕法尼亚大学（University of Pennsylvania）医学院，博士后/研究助理

2006-至今  武汉大学生命科学学院，教授

2010-2012  湖北省楚天学子

主要研究领域及兴趣

一些种类的真菌在碱性pH、高温、氮源缺乏等条件下能够从椭圆形的酵母形态转换成为菌丝或假菌丝，被称为二型性转换（dimorphic transition）。二型性转换被认为是真菌应对环境压力的一种反应，它也是某些致病性真菌的毒力因子之一。解脂耶氏酵母Yarrowia lipolytica是一种在进化上与酿酒酵母和白色念珠菌亲缘关系较远而与丝状真菌更为接近的酵母种类，对人无致病性，它具有强大的降解烷烃、脂肪酸和油脂的能力，在发酵工业中具有良好的应用前景。解脂耶氏酵母在葡萄糖等碳源存在时、碱性pH、低氧等条件下能够从椭圆形的酵母形态转换为菌丝或假菌丝，我们的研究兴趣是寻找参与调控解脂耶氏酵母二型性转换的基因，探索其作用机制，以增进对真菌二型性转换调控机制的认识。

主持课题项目

1.　国家自然科学基金面上项目（32070072），解脂耶氏酵母新型转录因子Fts1和Fts2负调控二型性转换的机制研究，2021-2024

2.　国家自然科学基金面上项目（31870062），酿酒酵母极性生长调控分子Boi2的功能机制研究，2019-2022

3.　国家自然科学基金面上项目（31570076），Ras信号传递途径下游效应分子Mhy1与YlRim15在解脂耶氏酵母二型性转换中的功能与调控机制研究，2016-2019

4.　国家自然科学基金面上项目（31370124），酿酒酵母极性生长关键调控分子Rho3的新调控因子、下游效应分子及功能的鉴定，2014-2017

5.　国家自然科学基金面上项目（30871347），酿酒酵母 Rho GTP酶在细胞极性建立过程中调控机制的研究，2009-2011

6.　国家自然科学基金面上项目（30770017），酿酒酵母Bud3p对septin细胞骨架调节机制的研究，2008-2010

发表的论文（*通讯作者）

1. Shu T, He X-Y, Chen J-W, Mao Y-S, Gao X-D* (2021) The pH-responsive transcription factors YlRim101 and Mhy1 regulate alkaline pH-induced filamentation in the dimorphic yeast Yarrowia lipolytica. mSphere 6(3): e00179-21.
2. Zhu J, Jia Z-W, Xia C-Y, Gao X-D* (2020) The Sur7/PalI family transmembrane protein Tos7 (Yol019w) plays a role in secretion in budding yeast. Fungal Genet. Biol. 144: 103467.
3. Jia Z-W, Lv S-X, Zhu J, Xia C-Y, Gao X-D* (2020) Roles of the PH, coiled-coil and SAM domains of the yeast polarity protein Boi2 in polarity-site localization and function in polarized growth. Curr. Genet. 66(6): 1101-1115.
4. Wu H, Shu T, Mao Y-S, Gao X-D* (2020) Characterization of the promoter, downstream target genes and recognition DNA sequence of Mhy1, a key filamentation-promoting transcription factor in the dimorphic yeast Yarrowia lipolytica. Curr. Genet. 66(1): 245-261.
5. Nie W-C, He F, Yuan S-M, Jia Z-W, Wang R-R, Gao X-D* (2017) Roles of an N-terminal coiled-coil-containing domain in the localization and function of Bem3, a Rho GTPase-activating protein in budding yeast. Fungal Genet. Biol. 99: 40-51.
6. Liang S-H, Wu H, Wang R-R, Wang Q, Shu T, Gao X-D* (2017) The TORC1-Sch9-Rim15 signaling pathway represses yeast-to-hypha transition in response to glycerol availability in the oleaginous yeast Yarrowia lipolytica. Mol. Microbiol. 104(4):553-567.
7. Yuan S-M, Nie W-C, He F, Jia Z-W, Gao X-D* (2016) Kin2, the budding yeast ortholog of animal MARK/PAR-1 kinases, localizes to the sites of polarized growth and may regulate septin organization and the cell wall. PLoS ONE 11(4): e0153992.
8. Wu H, Guo J, Zhou Y-T, Gao X-D* (2015) The anillin-related region of Bud4 is the major functional determinant for Bud4’s function in septin organization during bud growth and axial bud-site selection in budding yeast. Eukaryot. Cell 14(3): 241-251.
9. He F, Nie W-C, Tong Z, Yuan S-M, Gong T, Liao Y, Bi E, Gao X-D* (2015) The GTPase-activating protein Rga1 interacts with Rho3 GTPase and may regulate its function in polarized growth in budding yeast. PLoS ONE 10(4): e0123326.
10. Li Y-Q, Li M, Zhao X-F, Gao X-D* (2014) A role for the Rap GTPase YlRsr1  in cellular morphogenesis and the involvement of YlRsr1 and the Ras GTPase YlRas2 in bud-site selection in the dimorphic yeast Yarrowia lipolytica. Eukaryot. Cell 13(5): 580-590.
11. Li M, Li Y-Q, Zhao X-F, Gao X-D* (2014) Roles of the three Ras proteins in the regulation of dimorphic transition in the yeast Yarrowia lipolytica. FEMS Yeast Res. 14(3): 451-463.
12. Gong T, Liao Y, He F, Yang Y, Yang D-D, Chen X-D, Gao X-D* (2013) Control of polarized growth by the Rho family GTPase Rho4 in budding yeast: Requirement of the N-terminal extension of Rho4 and regulation by the Rho GTPase-activating protein Bem2. Eukaryot. Cell 12(2): 368-377.
13. Liao Y, He F, Gong T, Bi E, Gao X-D* (2013) Msb1 interacts with Cdc42, Boi1, and Boi2 and may coordinate Cdc42 and Rho1 functions during early stage of bud development in budding yeast. PLoS One 8:e66321.
14. Zhao XF, Li M, Li YQ, Chen XD, Gao X-D* (2013) The TEA/ATTS transcription factor YlTec1p represses the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica. FEMS Yeast Res. 13:50-61.
15. Tang Y, Gao X-D, Wang Y, Yuan BF, Feng YQ (2012) Widespread existence of cytosine methylation in yeast DNA measured by gas chromatography/mass spectrometry. Anal. Chem. 84:7249-55.
16. Guo J, Gong T, Gao X-D* (2011) Identification of an amphipathic helix important for the formation of ectopic septin spirals and axial budding in yeast axial landmark protein Bud3p. PLoS One 6:e16744.
17. Tong Z, Gao X-D, Howell AS, Bose I, Lew DJ, Bi E (2007) Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition. J. Cell Biol. 179:1375-1384.
18. Gao X-D, Sperber LM, Kane SA, Tong Z, Tong AHY, Boone C, Bi E (2007) Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle. Mol. Biol. Cell 18:2542-2560.
19. Tcheperegine SE, Gao X-D, Bi E (2005) Regulation of cell polarity by interactions of Msb3 and Msb4 with Cdc42 and polarisome components. Mol. Cell. Biol. 25:8567-8580.
20. Gao X-D, Caviston JP, Tcheperegine SE, Bi E (2004) Pxl1p, a paxillin-like protein in Saccharomyces cerevisiae, may coordinate Cdc42p and Rho1p functions during polarized growth. Mol. Biol. Cell 15:3977-3985.
21. Grimme SJ, Gao X-D, Martin PS, Tu K, Tcheperegine SE, Corrado K, Farewell AE, Orlean P, Bi E (2004) Deficiencies in the endoplasmic reticulum (ER)-membrane protein Gab1p perturb transfer of glycosylphosphatidylinositol to proteins and cause perinuclear ER-associated actin bar formation. Mol. Biol. Cell 15:2758-2770.
22. Gao X-D, Albert S, Tcheperegine SE, Burd CG, Gallwitz D, Bi E (2003) The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization. J. Cell Biol. 162:635-646.