学习经历

2004-2009  中国科学院物理研究所，博士 软物质物理与生物物理

2000-2004  山东大学物理学院，学士 物理学基地班

工作经历与任职情况

2020-至今   教授，博士生导师 武汉大学生命科学学院

2015-2020  研究员，博士生导师 武汉大学生命科学学院，武汉大学高等研究院

2010-2015  研究科学家 新加坡-MIT联合科研中心生物系统与微观力学实验室

2009-2010  专利审查员 国家知识产权局

荣誉与奖励

湖北省高层次人才青年项目入选者（2018）

国家高层次人才青年项目入选者（2022）

主要研究领域及兴趣

使用单分子磁镊技术和单分子FRET技术，揭示RNA 、DNA、蛋白质等生物大分子的动态结构及相互作用。使用结构生物信息学方法，揭示生物大分子的结构动态和功能机制。

DNA甲基化、单分子检测技术、转录异常导致的神经-肌肉退行性疾病是我们目前的研究重点。

主持课题项目：

国家高层次人才青年项目（2022）

国家自然科学基金面上项目（2017、2020）

国家自然科学基金青年项目（2018）

代表性论文(#为共同第一作者，*为通讯作者)

1. Xiao-Cong Zhao#, Hai-Long Dong#, Xiao-Lu Li, Hong-Yu Yang, Xue-Feng Chen, Liang Dai, Wen-Qiang Wu, Zhi-Jie Tan* and Xing-Hua Zhang*, “5-methyl-cytosine stabilizes DNA but hinders DNA hybridization revealed by magnetic tweezers and simulations.” Nucleic Acids Research, minor revision (2022) [IF 19.160]

2. Chen Zhang#, Fujia Tian#, Ying Lu, Bing Yuan, Zhi-Jie Tan, Xing-Hua Zhang* and Liang Dai*, “Twist-diameter coupling drives DNA twist changes with salt and temperature.” Science Advances, 8(12), abn1384 (2022) [IF 14.957]

3. Xiao-Wei Qiang#, Chen Zhang#, Hai-Long Dong#, Fu-Jia Tian, Hang Fu, Ya-Jun Yang, Liang Dai, Xing-Hua Zhang*, Zhi-Jie Tan*, “Multivalent cations reverse the twist-stretch coupling of RNA.” Physical Review Letters, 126, 108103. (2022) [IF 9.227]

4. Xuejie Wang#, Yang Dong#, Xiaocong Zhao#, Jinbao Li, Jordan Lee, Zhenxin Yan, Shuangshuang Yang, Wenqiang Wu, Ximiao Hou, Guangxue Liu, Yueyue Zhang, Lun Song, Gang Cai, Qing Li, Grzegorz Ira, Xinghua Zhang*, Xuefeng Chen*, “Rtt105 promotes high-fidelity DNA replication and repair by regulating the single-stranded DNA binding factor RPA.” PNAS, 118 (25): e2106393118 (2021) [IF 12.777]
5. Ya-Jun Yang, Hai-Long Dong, Xiao-Wei Qiang, Hang Fu, Er-Chi Zhou, Chen Zhang, Lei Yin, Xue-Feng Chen, Fu-Chao Jia, Liang Dai, Zhi-Jie Tan, and Xing-Hua Zhang*. “Cytosine methylation enhances DNA condensation revealed by equilibrium measurements using magnetic tweezers”. Journal of the American Chemical Society. 142 (20): 9203–9209 (2020) [IF 15.419]
6. Hang Fu#, Chen Zhang#, Xiao-Wei Qiang#, Ya-Jun Yang, Zhi-Jie Tan* and Xing-Hua Zhang*. “Opposite effects of high-valent cations on the elasticities of DNA and RNA duplexes revealed by magnetic tweezers.” Physical Review Letters. 124(5): 058101 (2020) [IF 9.227]
7. Chen Zhang#, Hang Fu#, Yajun Yang#, Erchi Zhou, Zhijie Tan, Huijuan You, Xinghua Zhang*. “The Mechanical Properties of RNA-DNA Hybrid Duplex Stretched by Magnetic Tweezers”. Biophysical Journal. 116 (2): 196-204 (2019) [IF 4.033, 期刊年度最佳论文]
8. Ya-Jun Yang#, Lun Song, Xiao-Cong Zhao, Chen Zhang, Wen-Qiang Wu, Hui-Juan You, Hang Fu, Er-Chi Zhou, Xing-Hua Zhang*. “A Universal Assay for Making DNA, RNA, and RNA–DNA Hybrid Configurations for Single-Molecule Manipulation in Two or Three Steps without Ligation”. ACS synthetic biology. 8(7): 1663-1672 (2019) [IF 5.110]
9. Ju-Hui Liu #, Kun Xi #, Xi Zhang, Lei Bao, Xing-Hua Zhang*, Zhi-Jie Tan*. “Structural Flexibility of DNA-RNA Hybrid Duplex: Stretching and Twist-Stretch Coupling”. Biophysical Journal. 117 (1): 74-86 (2019) [IF 4.033]
10.  Xiao-Cong Zhao #, Hang Fu, Lun Song, Ya-Jun Yang, Er-Chi Zhou, Guang-Xue Liu, Xue-Feng Chen, Zhuo Li, Wen-Qiang Wu, Xing-Hua Zhang*, “S‑DNA and RecA/RAD51-Mediated Strand Exchange in Vitro”. Biochemistry. 58 (15):  2009-2016 (2019) [IF 3.162]
11. Xinghua Zhang, Yuanyuan Qu, Hu Chen, Ioulia Rouzina, Shengli Zhang, Patrick S. Doyle*, and Jie Yan*, “Interconversion between three overstretched DNA structures.” Journal of the American Chemical Society, 136(45): 16073-16080 (2014) [IF 15.419]
12.  Xinghua Zhang, Hu Chen, Shimin Le, Ioulia Rouzina, Patrick S. Doyle*, and Jie Yan*, “Revealing the competition between peeled ssDNA, melting bubbles and S-DNA during DNA overstretching by single-molecule calorimetry”, PNAS, 110(10): 3865-3870 (2013) [IF 12.777]
13. Xinghua Zhang, Hu Chen, Hongixa Fu, Patrick S. Doyle*, and Jie Yan*, "Two distinct overstretched DNA structures revealed by single-molecule thermodynamics measurements", PNAS, 109 (21): 8103-8108 (2012) [IF 12.777]