学习经历

1996-2001  日本京都大学大学院工学研究科合成及生物化学专业 博士

1989-1992  武汉大学生命科学学院微生物学专业 硕士

1985-1989  武汉大学生物系微生物学专业 学士

工作经历

2003-至今    武汉大学生命科学学院微生物学系 副教授、教授

2001-2003  日本国立产业技术综合研究所生物情报解析研究中心博士后 (JSPS Fellow)

1992-1996  武汉大学生命科学学院微生物学系 助教、讲师

教学获奖

1. 2013年湖北省高等学校教学成果一等奖（排名二）

2. 2008年国家级教学团队（微生物学系列课程教学团队主要成员）

3. 2005年国家级教学成果二等奖（排名第三）

4. 2005年湖北省高等学校教学成果一等奖（排名第三）

主持和参加课题

主持国家自然科学基金、教育部留学回国人员科研启动基金等，参加973项目子项目。主持武汉大学教改项目1项，参加多项国家级、省部级教改项目。

● 国家自然科学基金（31570062）嗜盐古菌Tat及Sec信号肽在胞外蛋白酶折叠、成熟及转运过程中的作用差异性的分子机制

● 国家自然科学基金（30970071）嗜盐古菌胞外蛋白酶分泌机制的研究

● 国家自然科学基金（30870063）嗜盐古菌蛋白酶SptA的C端延伸区功能研究

● 国家自然科学基金（30470019）极端嗜热古菌蛋白酶Pyrolysin插入序列功能的研究

主要社会兼职

中国微生物学会微生物学教学工作委员会委员（2016-2021）

《微生物学通报》编委（2016-2021）

中国微生物学会会员

美国微生物学会 (American Society for Microbiology) 会员

中国遗传学会会员

发表的论文（*通讯作者）

1. Li M, Yin J, Mei S, Wang X,Tang XF*,Tang B* (2018) Halolysin SptA, a serine protease, contributes to growth-phase transition of haloarchaeonNatrinemasp. J7-2, and its expression involves cooperative action of multiplecis-regulatory elements.Front Microbiol. 9:1799.

2. Perveen S, Rashid N,Tang XF, Imanaka T, Papageorgiou AC* (2017) Anthranilate phosphoribosyltransferase from the hyperthermophilic archaeon Thermococcus kodakarensis shows maximum activity with zinc and forms a unique dimeric structure.FEBS Open Bio. 7(8):1217-30.

3. Gao X, Zeng J, Yi H, Zhang F, Tang B*,Tang XF* (2017) Four inserts within the catalytic domain confer extra stability and activity to hyperthermostable pyrolysin fromPyrococcus furiosus. Appl Environ Microbiol83(5).pii:e03228-16.

4. Zhu F,Yang X, Wu Y, Wang Y,Tang XF, Tang B* (2017) Release of an HtrA-like protease from the cell surface of thermophilic Brevibacillus sp. WF146 via substrate-induced autoprocessing of the N-terminal membrane anchor.Front Microbiol8:481.

5. Tang W, Wu Y, Li M, Wang J, Mei S, Tang B*,Tang XF* (2016) Alternative translation initiation of a haloarchaeal serine protease transcript containing two in-frame start codons.J Bacteriol. 198(13):1892-901. (Spotlight article)

6. Liu F, Zhao ZS, Ren Y, Cheng G,Tang XF, Tang B* (2016) Autocatalytic activation of a thermostable glutamyl endopeptidase capable of hydrolyzing proteins at high temperatures.Appl Microbiol Biotechnol100(24):10429-41.

7. Meng D, Dai M, Xu BL, Zhao ZS, Liang X, Wang M,Tang XF, Tang B* (2016) Maturation of fibrinolytic bacillopeptidase F involves both hetero- and autocatalytic processes.Appl Environ Microbiol82(1):318-27.

8. Xu BL, Dai M, Chen Y, Meng D, Wang Y, Fang N,Tang XF, Tang B* (2015) Improving the thermostability and activity of a thermophilic subtilase by incorporating structural elements of its psychrophilic counterpart.Appl Environ Microbiol81(18):6302-13.

9. Wang L, Cheng G, Ren Y, Dai Z, Zhao ZS, Liu F, Li S, Wei Y, Xiong J,Tang XF, Tang B* (2015) Degradation of intact chicken feathers by Thermoactinomyces sp. CDF and characterization of its keratinolytic protease.Appl Microbiol Biotechnol99(9): 3949-59.

10. Du X, Li M, Tang W, Zhang Y, Zhang L, Wang J, Li T, Tang B,Tang XF* (2015) Secretion of Tat-dependent halolysin SptA capable of autocatalytic activation and its relation to haloarchaeal growth.Mol Microbiol96(3):548-65.

11. Zhang Y, Wang M, Du X, Tang W, Zhang L, Li M, Wang J, Tang B,Tang XF* (2014) Chitin accelerates activation of a novel haloarchaeal serine protease that deproteinizes chitin-containing biomass.Appl Environ Microbiol80(18):5698-708.

12. Zeng J, Gao X, Dai Z, Tang B,Tang XF* (2014) Effects of metal ions on stability and activity of hyperthermophilic pyrolysin and further stabilization of this enzyme by modification of a Ca2+-binding site.Appl Environ Microbiol80(9):2763-72.

13. Feng J, Wang J, Zhang Y, Du X, Xu Z, Wu Y, Tang W, Li M, Tang B,Tang XF* (2014) Proteomic analysis of the secretome of haloarchaeon Natrinema sp. J7−2.J Proteome Res13(3):1248-58.

14. Zhu H, Xu BL, Liang X, Yang YR,Tang XF, Tang B* (2013) Molecular basis for auto- and hetero-catalytic maturation of a thermostable subtilase from thermophilic Bacillus sp. WF146.J Biol Chem288(48):34826-38.

15. Li XG*, Yang J, Wang R,Tang XF, Meng JJ, Qin HJ, Liu X, Guo F, Wan SB* (2013) Effects of ectopically expressed hyperthermophilic archaeon (Pyrococcus furiosus) ribulose-1,5-bisphosphate carboxylase/oxygenase on tobacco photosynthesis.Photosynthetica51 (3): 387-94.

16. Feng J, Liu B, Zhang Z, Ren Y, Li Y, Gan F, Huang Y, Chen X, Shen P, Wang L, Tang B*,Tang XF* (2012) The complete genome sequence of Natrinema sp. J7-2, a haloarchaeon capable of growth on synthetic media without amino acid supplements.PLoS ONE7(7):e41621.

17. Dai Z, Fu H, Zhang Y, Zeng J, Tang B,Tang XF* (2012) Insights into the maturation of hyperthermophilic pyrolysin and the roles of its N-terminal propeptide and long C-terminal extension.Appl Environ Microbiol78(12):4233-41.

18. Xu Z, Du X, Li T, Gan F, Tang B,Tang XF* (2011) Functional Insight into the C-Terminal Extension of Halolysin SptA from Haloarchaeon Natrinema sp. J7.PLoS ONE6(8):e23562.

19. Liang X, Bian Y,Tang XF, Xiao G, Tang B* (2010) Enhancement of keratinolytic activity of a thermophilic subtilase by improving its autolysis resistance and thermostability under reducing conditions.Appl Microbiol Biotechnol87:999–1006.

20. Fang N, Zhong CQ, Liang X,Tang XF, Tang B* (2010) Improvement of extracellular production of a thermophilic subtilase expressed in Escherichia coli by random mutagenesis of its N-terminal propeptide.Appl Microbiol Biotechnol85(5):1473-81.

21. Zhong CQ, Song S, Fang N, Liang X, Zhu H,Tang XF, Tang B* (2009) Improvement of low-temperature caseinolytic activity of a thermophilic subtilase by directed evolution and site-directed mutagenesis.Biotechnol Bioeng104(5):862-70. (Spotlight article)

22. Cheng G, Zhao P,Tang XF, Tang B* (2009) Identification and characterization of a novel spore-associated subtilase from Thermoactinomyces sp. CDF.Microbiology-SGM55:3661-72.

23. Zeng C, Zhao YZ, Cui CZ, Zhan H, Zhu JY,Tang XF, Shen P, Huang YP*, Chen XD*. (2009) Characterization of the Haloarcula hispanica amyH gene promoter, an archaeal promoter that confers promoter activity in Escherichia coli.Gene442: 1–7.

24. Yang YR, Zhu H, Fang N, Liang X, Zhong CQ,Tang XF, Shen P, Tang B* (2008) Cold-adapted maturation of thermophilic WF146 protease by mimicking the propeptide binding interactions of psychrophilic subtilisin S41.FEBS Lett582(17):2620-6.

25. Shi W,Tang XF, Huang Y, Gan F, Tang B*, Shen P（2006）An extracellular halophilic protease SptA from a halophilic archaeon Natrinema sp. J7: gene cloning, expression and characterization.Extremophiles10:599–606.

26. Bian Y, Liang X, Fang N,Tang XF, Tang B*, Shen P, Peng Z（2006）The roles of surface loop insertions and disulfide bond in the stabilization of thermophilic WF146 protease.FEBS Lett580:6007–14. (Faculty 1000 of Biology Recommended)

27. Tang XF, Shen Y, Matsui E, Matsui I* (2004) Domain topology of the DNA polymerase D complex from a hyperthermophilic archaeon Pyrococcus horikoshii.Biochemistry43(37):11818-27.

28. Shen Y,Tang XF, Yokoyama H, Matsui E, Matsui I* (2004) A 21-amino acid peptide from the cysteine cluster II of the family D DNA polymerase from Pyrococcus horikoshii stimulates its nuclease activity which is Mre11-like and prefers manganese ion as the cofactor.Nucleic Acids Res32:158-68.

29. Shen Y,Tang XF, Matsui E, Matsui I* (2004) Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerase from Pyrococcus horikoshii.Biochem Soc Trans32:245-9.

30. Shen Y,Tang XF, Matsui I* (2003) Subunit interaction and regulation of activity through terminal domains of family D DNA polymerase from Pyrococcus horikoshii.J Biol Chem278: 21247-57.

31. Tang XF, Ezaki S, Atomi H, Imanaka T* (2001) Anthranilate synthase without an LLES motif from a hyperthermophilic archaeon is inhibited by tryptophan.Biochem Biophys Res Commun281:858-65.

32. Matsuda T, Fujikawa M, Haruki M,Tang XF, Ezaki S, Imanaka T, Morikawa M, Kanaya S* (2001) Interaction of TIP26 from a hyperthermophilic archaeon with TFB/TBP/DNA ternary complex.Extremophiles5:177-82.

33. Tang XF, Ezaki S, Atomi H, Imanaka T* (2000) Biochemical analysis of a thermostable tryptophan synthase from a hyperthermophilic archaeon.Eur J Biochem267:6369-77.

34. Tang XF, Ezaki S, Fujiwara S, Takagi M, Atomi H, Imanaka T* (1999) The tryptophan biosynthesis gene cluster trpCDEGFBA from Pyrococcus kodakaraensis KOD1 is regulated at the transcriptional level and expressed as a single mRNA.Mol Gen Genet262:815-21.